Based on the strong modeling and simulation, analysis and calculation of satellite tool kit (STK), the connection interface between Matlab and STK is studied. The communication model between Matlab and STK is established. The simulation and analysis of STK and Matlab are applied to Walker constellation design and optimization. Realized connect between Matlab and STK, object creation and data extraction functions, and data visualization processing. The results show that the change of orbit inclination has a great influence on the searching time, but the change of the orbital plane number has little effect on the searching time. The interactive interface of Walker constellation design can provide convenient and effective support platform for space mission simulation and analysis.