According to the requirements of flow field performance test in the vertical test section of vertical wind tunnel, a calibration frame system based on double-tower structure is designed. The full closed loop AC servo control system makes the upper and lower screw connecting plates drive the rack to do high precision synchronous or single-step positioning control; the space positioning is realized by cooperating with the control system, and the calibration of parameters such as directional field, dynamic pressure field and axial static pressure gradient is realized by the measurement system composed of directional dynamic pressure combination probe and DSM electronic scanning valve. The results show that when v = 35 m/s, the qualified area of each section is more than 75%. The system has successfully completed the task of vertical wind tunnel flow field calibration, the measurement data is accurate, and the calibration results meet the requirements of the national military standard.