Frequency planning and optimizing station distributing are researched to decrease the interference between stations in small region in complex electromagnetic environment. The method of frequency selection of station in complex electromagnetic environment was researched, with four kinds of interference between receivers analyzed. Genetic algorithm is used to design optimum distribution of stations which use the same frequency in the same time. The optimum distribution of 7 stations was given by using matlab software. The solution calculated by genetic algorithm can decrease the interference in certain degree and can provide reference when distributing stations in small area.