A signal acquisition card for launch vehicle multi-channel pulse signal is designed according to the characteristics and requirements of launch vehicle ground testing. Acquisition card uses the FPGA chip counter to transport acquired real-time multi-channel pulse signal to upper PC through CPCI bus. The acquisition card is tested, the results show that the design scheme satisfied the requirements. Compared with the current launch vehicle pulse signal ground test equipment, the designed card has the advantage of smaller size, higher integration and reliability, less resource expense, etc.