In order to obtain accurate pedestal oblique quantity, a method of demarcating measuring unit of the pedestal obliquity measuring system of gyroscope based on the least squares method was put forward. The essential principle of pedestal obliquity measuring system of gyroscope based on electrolyte obliquity sensor was introduced. The value and direction of the pedestal oblique were measured accurately. According to the data model between pedestal oblique and north-seeking precision effect, the north-seeking error was compensated. And the data were fitted according to the data model established by the least squares method. When the discrepancy between the measuring unit demarcated and theoretical design value was great, the unit could be demarcated again by adjusting the circuit magnification multiple. When the measuring unit demarcated was accord with the theoretical design value, the measuring unit demarcated was recorded and written into the flash unit of single-Chip microcomputer. Analysis result showed that measuring unit could be demarcated fast and easy by this measuring method, and the precision of north-seeking could be ensured.